Reporter

Part:BBa_K2429049:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


pTRE mKate FF4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 1495
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 1495
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 1495
    Illegal BamHI site found at 339
    Illegal XhoI site found at 775
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 1495
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 1495
    Illegal XbaI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400
    Illegal SapI.rc site found at 393


Design Notes

Ensure that the intron can be efficient spliced out of the construct.



Source

Part received from Weiss Lab


References